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Glycolysis, Pi G3P dehydrogenase 2x NADH + H+, 2x 2-Phosphoglycerate Enolase 2x H2O, ATP Phosphofructokinase Fructose-1,6-bisphosphate, Fructose-6-phosphate Phosphofructokinase This is the main regulatory step, being the first step commited to this pathway. It is increased by AMP and Fructose-2,6-bisphosphate. It is inhibited by ATP, citrate and fatty acids., 2x 1,3-bisphosphoglycerate Phosphoglycerate kinase 2x 3-Phosphoglycerate, ATP Phosphofructokinase This is the main regulatory step, being the first step commited to this pathway. It is increased by AMP and Fructose-2,6-bisphosphate. It is inhibited by ATP, citrate and fatty acids., 2x 3-Phosphoglycerate phosphoglyceromutase 2x 2-Phosphoglycerate, 2x Glyceraldehyde-3-phosphate G3P dehydrogenase 2x NADH + H+, ATP Phosphofructokinase ADP, Glucose hexokinase ADP